Biggs, Danielle L., Corinne S. Lengsfeld, Brooks M. Hybertson, Lawrence K. Ng, Mark C. Manning, and Theodore W. Randolph. CELLULAR RESPONSE TO POLY(L-LACTIDE) MICROPARICLES FOR PULMONARY DELIVERY
4180 Evans Dr., Boulder, CO 80303 (biggs@ucsub.colorad)
P(L)LA microspheres produced using the precipitation-with-a-compressed-antisolvent (PCA) process were used to study alveolar cellular response to microparticles both in vitro and in vivo. Microspheres were produced by spraying a P(L)LA/organic solution into supercritical CO2. The in vitro response to P(L)LA microparticles was studied using an alveolar macrophage cell line (NR8383), and the in vivo response was studied after intratracheal instillation of microparticles in guinea pigs. In vitro reactive oxygen species production was measured to quantify cellular response using both fluorescence and chemiluminescence. The in vivo cellular response was investigated by analyzing recovered bronchialveolar lavage fluid for inflammatory cells and precursors.
Results show exposure of NR8383 cells to microspheres of different crystallinity elicited a significantly different cell response, which was practically negligible when compared to the high response induced by the positive control.