Planetary Metabolism Symposium
     February 21, 2003  •  University of Colorado at Boulder
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Factors Affecting In-Stream Denitrification Rates in a Nitrogen-Contaminated Stream in the Upper Illinois River Basin: A Comparison of Two Techniques

L.K. Smith1, R. Antweiler2, J.K. Bohlke3, J. Harvey3,
R.L. Smith2 and M.A.Voytek3

1CIRES, University of Colorado, Boulder, CO
2USGS, Boulder, CO
3USGS, Reston, VA

Denitrification is usually considered one of the most important processes controlling nitrogen loads in streams and rivers because it has the capability of permanently removing fixed nitrogen. Elevated loads of nitrogen species are a common occurrence in rivers draining agricultural watersheds in the Midwestern United States. Few studies have been conducted in this region to determine the controls of in-stream nitrogen transformations, such as denitrification and nitrification, and their net effect on nitrogen transport in small and medium sized rivers. Current thinking suggests that nitrogen cycling processes should have the greatest impact in small rivers due to greater contact between river water and the streambed. The goal of this project is to evaluate the influence of channel type, hyporheic exchange, microbial community structure, and water column chemistry on two rivers located in the Upper Illinois River Basin. Furthermore, two different methods, membrane inlet and isotope ratio mass spectrometry, are being compared to measure dentirification rates in the laboratory.