Cooperative Institute for Research in Environmental Sciences

Bacteria from dog feces pervades winter air of urbanized areas

Bacteria from dog feces pervades winter air of urbanized areas


Bacteria from fecal material—in particular, dog fecal material—may constitute the dominant source of airborne bacteria in Cleveland’s and Detroit’s wintertime air, says a new study led by researchers from the Cooperative Institute for Research in Environmental Sciences (CIRES).

The study, which was published July 29 in Applied and Environmental Microbiology, showed that of the four Midwestern cities in the experiment, two cities had significant quantities of fecal bacteria in the atmosphere—with dog feces being the most likely source.

“We found unexpectedly high bacterial diversity in all of our samples, but to our surprise the airborne bacterial communities of Detroit and Cleveland most closely resembled those communities found in dog poop,” said lead author Robert Bowers, a CIRES graduate student in CU Boulder’s Ecology and Evolutionary Biology department. “This suggests that dog poop may be a potential source of bacteria to the atmosphere at these locations.”

Scientists already knew that bacteria exist in the atmosphere and that these bacteria can have detrimental effects on human health, triggering allergic asthma and seasonal allergies, said CIRES Fellow Noah Fierer, who is also an assistant professor in CU Boulder’s Ecology and Evolutionary Biology department. But it is only in recent years that researchers have realized that there is an incredible diversity of bacteria residing in the air, he said.

“There is a real knowledge gap,“ Fierer said. “We are just starting to realize this uncharted microbial diversity in the air—a place where you wouldn’t exactly expect microbes to be living.”

To gain further understanding of just what microbes are circulating in urban environments, the team analyzed the local atmosphere in the summer and winter at four locations in the Great Lakes region of the U.S. Three of the locations—Chicago, Cleveland and Detroit—are major cities with populations greater than 2 million, and one location, Mayville, WI, is a small town with a population of less than 6,000.

The team used nearly 100 air samples, collected as part of a previous study, conducted by Colorado State University (CSU). The CSU experiment investigated the impacts of biomass burning (burning of trees or plant material) on air quality and involved studying the effect of residential wood burning and prescribed fires on airborne fine particle concentrations in the midwestern United States.

“What we’ve been looking at are the numbers and the types of bacteria in the atmosphere,” Fierer said. “We breathe in bacteria every minute we are outside, and some of these bugs may have potential health implications.”

The researchers analyzed the bacteria’s DNA in the collected air samples and compared the bacteria found against a database of bacteria from known sources such as leaf surfaces, soil and human, cow and dog feces. They discovered that the bacterial “communities” in the air were surprisingly diverse and also that, in two of the four locations, dog feces were a greater than expected source of bacteria to the atmosphere in the wintertime.

In the summer, airborne bacteria come from many sources—soil, dust, leaf surfaces, lakes and oceans, Bowers said. But in the winter, as leaves drop and snow covers the ground, the influence that these environments have as sources also goes down. It is during this season that the airborne communities appeared to be more influenced by dog feces than the other sources tested in the experiment, he said.

“As best as we can tell, dog feces are the only explanation for these results,” Fierer said. “But we do need to do more research.”

The team plans to investigate the bacterial communities in other cities and build a continental-scale atlas of airborne bacterial communities, Fierer said. “We don’t know if the patterns we observed in those sites are unique to those cities,” he said. “Does San Francisco have the same bacteria as New York? Nobody knows as yet.”

Fierer believes it is important to pin down the types of bacteria in the air; how these bacteria vary by location and season; and where they are coming from. With this information, scientists would then be able to investigate the possible impacts on human health, he said.

“We need much better information on what sources of bacteria we are breathing in every time we go outside,” Fierer said.

Co-authors on the study included Rob Knight an associate professor in CU Boulder’s Department of Chemistry and Biochemistry, Amy Sullivan and Jeff Collett Jr. of Colorado State University, and Elizabeth Costello of Stanford University School of Medicine. The study was funded by the CIRES Innovative Research Program, the U.S. Environmental Protection Agency, the National Science Foundation, the Howard Hughes Medical Institute and the National Institutes of Health. The Lake Michigan Air Directors Consortium (LADCO) supported the aerosol sample collection for the project.

Noah Fierer, CIRES, 303-492-5615, Noah.Fierer@colorado.edu

Jeff Collett, CSU, 970-491-8697, collett@atmos.colostate.edu

Kathleen Human, CIRES, 303-492-6289, kathleen.human@colorado.edu


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