Visiting Fellow Seminar: Uwe Karst
An image says more than a thousand words: Mass spectrometric imaging and complementary techniques in pharmaceutical and biomedical analysis
by Uwe Karst, University of Münster, Institute of Inorganic and Analytical Chemistry
Abstract: Elemental bioimaging by means of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) or microscale X-ray fluorescence spectroscopy (µXRF) is a capable strategy for the spatially resolved investigation of metals in biological samples. The high sensitivity and excellent detection limits of LA-ICP-MS and the non-destructive nature of µXRF allow the determination of elements in low concentration ranges in various samples, typically in thin tissue slices. Complementary molecular information is provided by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging.
This presentation will focus on the general strategies and recent examples, including the diagnosis of Wilson´s disease from human liver biopsy samples, studies of the fate of instilled nanoparticles in the lung of rats, and the spatialy resolved analysis of metals from implants deposited in the human body.
Bio: Uwe Karst obtained his Ph.D. in Analytical Chemistry at the University of Münster, Germany in 1993. He moved to the University of Colorado in Boulder, CO, USA as a postdoctoral research associate with Bob Sievers. After his return to the University of Münster, he finished his Habilitation in 1998. In 2001, he took over a position as Full Professor of Chemical Analysis at the University of Twente, The Netherlands. He accepted his current position as Chair of Analytical Chemistry at the University of Münster in 2005. His research interests focus on hyphenated analytical techniques and their (bio)medical and pharmaceutical applications, including elemental speciation analysis, metallomics, mass spectrometric imaging and electro-chemistry/MS. Currently, Uwe is CIRES Visiting Fellow at the University of Colorado.